Question 1

What is the HB Electrophoresis test used for?

Accepted Answer

The Hemoglobin (HB) Electrophoresis test is primarily used to identify and measure different types of hemoglobin in the blood. It is the gold standard diagnostic tool for detecting inherited hemoglobin disorders, collectively known as hemoglobinopathies. These include Beta Thalassemia (both Minor/Trait and Major), Alpha Thalassemia variants, Sickle Cell Disease, Sickle Cell Trait, and other rare hemoglobin variants like Hemoglobin C, E, and D. It is also widely used for pre-marital screening, prenatal diagnosis, and evaluating unexplained chronic microcytic anemia.

Question 2

Do I need to fast before an HB Electrophoresis test?

Accepted Answer

No, fasting is not required for a Hemoglobin Electrophoresis test. You can eat and drink normally before the sample collection. However, it is highly critical to inform the laboratory staff and your physician if you have received a blood transfusion within the last 3 to 4 months. Blood transfusions introduce donor red blood cells into your circulation, which can temporarily mask your native hemoglobin pattern and lead to inaccurate or misleading test results.

Question 3

How does a recent blood transfusion affect my test results?

Accepted Answer

A blood transfusion introduces normal adult hemoglobin (HbA) from a healthy donor into your bloodstream. If you have an underlying hemoglobinopathy, such as Thalassemia Major or Sickle Cell Disease, the presence of donor red blood cells will dilute your abnormal hemoglobin variants and falsely elevate your normal hemoglobin levels. This can lead to a false-negative or inconclusive result. To ensure diagnostic accuracy, it is medically recommended to wait at least 120 days (4 months) after your last blood transfusion before undergoing this test.

Question 4

What is the difference between Thalassemia Minor and Thalassemia Major on this test?

Accepted Answer

On an HB Electrophoresis test, Thalassemia Minor (or Beta Thalassemia Trait) is typically characterized by a mild elevation of Hemoglobin A2 (usually between 3.5% and 8.0%) and sometimes a slight increase in Hemoglobin F, while Hemoglobin A remains the predominant type. In contrast, Thalassemia Major is characterized by a severe reduction or complete absence of Hemoglobin A, with Hemoglobin F making up the vast majority (often 70% to 95%) of the total hemoglobin, accompanied by severe clinical symptoms of anemia.

Question 5

How can I access my HB Electrophoresis report from Chughtai Lab?

Accepted Answer

Chughtai Lab offers multiple convenient methods for patients to access their diagnostic reports. Once your HB Electrophoresis test is processed and verified by our consultant hematologists, you will receive an SMS notification. You can download your report directly from the official Chughtai Lab website by entering your patient ID and password, access it via the Chughtai Lab Mobile App, receive it automatically on WhatsApp, or collect a printed copy from any of our nationwide collection centers.

HB Electrophoresis Test in Pakistan at Chughtai Lab

Chughtai Lab

Frequently Asked Questions

HB Electrophoresis Test in Pakistan at Chughtai Lab

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Chughtai Lab

Frequently Asked Questions